High Ssensitivity Microcystins ELISA Test Kit

Microcystins ELISA Test Kit

CH 98018

Product Description

     The Microcystins ELISA is an immunoassay for the quantitative and sensitive congener-independent detection of Microcystins and Nodularins in water samples.

Sample Collection and Handling

 Collect water samples in glass or PETG containers and test within 24 hours. Use of other types of plastic collection and/or storage containers may result in adsorptive loss of Microcystins, producing inaccurate (falsely low) results. Drinking water samples should be treated with sodium thiosulfate immediately after collection . If samples must be held for longer periods (up to 5 days), samples should be stored refrigerated. For storage periods greater than 5 days, samples should be stored frozen.

 If total Microcystins concentration (free and cell bound) is required, an appropriate cell lysing procedure must be performed prior to analysis.

Note: The use of sonication in cell lysing can negatively affect toxin concentrations, producing falsely low sample results.

 Samples may be filtered prior to analysis using glass fiber filters. The use of alternate filter types (non-glass fiber filters) may produce falsely low sample results, as Microcystins may bind to the filter material, removing it from the sample. Also, please note that some glass fiber filters are manufactured using a process which may cause interference which would cause inaccurate (falsely high) results. To avoid this potential bias in sample results, a total volume of at least 10 mL should be passed through the glass fiber filter, with the first 5 mL of filtered sample being discarded and the second 5 mL collected for testing . If determining total Microcystins concentration, samples should be lysed prior to filtration to prevent the removal of cell-bound Microcystins, which would cause inaccurate (falsely low) results.